Extended Data Fig. 5: cCol I–DDR1–NRF2 signalling controls macropinocytosis and mitochondrial protein expression.
From: Collagenolysis-dependent DDR1 signalling dictates pancreatic cancer outcome
a, IB analysis of ETC complexes I-V (CI-CV) in Paren. and E79Q KPC cells plated on WT or R/R ECM and incubated in LG for 24 h. b, IB analysis of indicated KPC cells −/+ ectopic p62 or E79Q plated on WT ECM and incubated in LG for 24 h. c, IB of ETC proteins in Paren., DDR1Δ, or E79Q/DDR1Δ KPC cells plated on WT ECM and incubated in LG for 24 h. d,e, IB of indicated proteins in Paren. or E79Q KPC cells plated on WT ECM and incubated in LG medium −/+ML120B for 24 h. f, Staining intensity of the indicated proteins in tumour areas depicted Fig. 4e determined with Image J. g, IHC of liver sections prepared 2 wk after i.s. transplantation of KPC cells into CCl4 pretreated Col IWT and Col Ir/r mice. Scale bars, 100 μm. Image J determined staining intensity of indicated proteins in tumour areas is shown at the bottom. Results in (f,g) (n = 6 fields) are mean ± s.e.m. Statistical significance determined by two-tailed t-test. ****P < 0.0001.
