Extended Data Fig. 3: Microfluidic workflow for measuring the dependence of the separatrix on the concentration of reagents.
From: Nonlinear decision-making with enzymatic neural networks
a, We determined the equation of the separatrix in the (X1,X2) plane for 3 conditions in a multiplexed experiment. We first performed 3 rounds of droplet generation. During each round, we scanned the (X1,X2) plane by mixing 3 tubes (which all derive from the same master mix) and collected the emulsion in a separate tube. Between each round, we changed the set of 3 tubes - thus changing their common mastermix. This allowed us to vary the concentrations of converter templates or drain templates in the master mix. After generation, we simultaneously imaged the three subpopulations together. To that end, we sequentially and gently filled a chamber with each population: the subpopulations remained spatially separated. We then incubated and imaged the chamber. During image processing, we separated the subpopulation by selecting 3 distinct regions (shown as red boxes in the right picture).
