Extended Data Fig. 3: Cryo-electron microscopy (Cryo-EM) analysis of apo PsChs1.

a, Flowchart of cryo-EM data processing for apo-PsChs1. b, Gold-standard Fourier shell correlation (FSC) curves of apo-PsChs1, including FSC between two independently refined half-maps generated by RELION (blue) and model-to-map FSC generated by Phenix (red). c, Angular distribution of the particles contributing to the final reconstruction of PsChs1. d, The map of PsChs1 is colored according to local resolution estimation. e, Sample maps of TM helices, IF helices, N-terminal subdomains, and eight conserved motifs of apo-PsChs1.