Extended Data Fig. 3: Differential effects of cGAMP on IL-35 and IL-10 induction are compared in B and T cells.
From: STING-induced regulatory B cells compromise NK function in cancer immunity
a, Representative histograms of EBI3 (left), p35 (middle) and IL-10 (right) with geometric mean fluorescence intensity (gMFI) are shown. B and T cells were sorted from spleens of KPC4662 tumour-bearing or non-tumour bearing mice. B cells were stimulated with anti-IgM. T cells were stimulated with anti-CD3/anti-CD28. Cells were treated with or without cGAMP and analyzed by flow cytometry analysis at 24 hr post treatment. b, Quantification of the percentages of IL-35+ (top) and IL-10+ (bottom) cells in live CD19+ B cells and CD3+ T cells (n = 4, representative of two independent experiments). c, Treg(Ebi3+/−) and Treg(Ebi3−/−) mice orthotopically inoculated with KPC4662 cells and treated with cGAMP. Representative images of tumours (left) and tumour weights (right) at d21 are shown (n = 8 Ctrl PBS, n = 8 Ctrl cGAMP, n = 9 Treg(Ebi3−/−) PBS, n = 9 Treg(Ebi3−/−) cGAMP). Representative of two replicate studies (Extended Data Fig. 3a–c) are shown, with each symbol representing an individual animal and mean ± s.e.m. denoted. Statistical tests of two-way ANOVA (Extended Data Fig. 3b) and unpaired two-tailed Student’s t tests (Extended Data Fig. 3c) were performed. P values indicated; ns, not significant.
