Extended Data Fig. 4: Classification and refinement of ribosomes in native untreated cells.
From: Visualizing translation dynamics at atomic detail inside a bacterial cell

a, A diagram of the image-processing workflow. The classification process can be grouped into three tiers: global classification, focused classification on the tRNA path region (mask I), and focused classification on elongation factor and A/T tRNA binding region (mask II). For each class, a unique number identifier and class name are assigned for tracking. The particle number and the global map resolution at FSC = 0.143 are provided for each class. b-d, FSC curves for all refined maps.