Extended Data Fig. 10: rAPI5 protects Atg16L1ΔPC mice against DSS-induced intestinal inflammation.
From: The γδ IEL effector API5 masks genetic susceptibility to Paneth cell death
Atg16L1f/f (f/f) and Atg16L1ΔPC (ΔPC) mice were treated with 5% DSS with or without injection of rAPI5 (either rAPI5WT or rAPI5Y8K; Y11K) on day 0, 3, and 6, and euthanized on day 8. n = 5 (f/f), 5 (ΔPC), 7 (f/f rAPI5WT), and 6 (ΔPC rAPI5WT), 5 (f/f rAPI5Y8K; Y11K), and 5 (ΔPC rAPI5Y8K; Y11K). a, Quantification of TNFα in SN harvested from gut explants. b, c, d, e, Representative images of H&E staining (b and d) and quantification of villi length (c) and Paneth cells (e). Scale bar 100 μm (b) and 20 μm (e). An ANOVA with Tukey’s multiple-comparison test in (a, c, e). Data points in (b), (c), and (e) are individual mice. Bars represent means ± SEM, and at least two independent experiments were performed.
