Extended Data Fig. 2: PCA and Heatmaps for DEGs represented in Fig. 1.
From: Prenatal immune stress blunts microglia reactivity, impairing neurocircuitry
a, Principal component analysis (PCA) of CON and MIA microglia after SAL or LPS treatment. b, Top 25 DEGs between MIA and CON microglia after SAL treatment. c, Top DEGs between MIA and CON microglia after LPS treatment. Blunted LPS-induced genes and blunted LPS-repressed genes are indicated. d, Top DEGs between MIA and CON microglia that show an interaction between MIA and LPS (group x treatment). Blunted LPS-induced and LPS-repressed genes are indicated. e, Linear regression of the FC for the LPS-induced DEGs from Figure 1e of MACS-isolated microglia (left) in comparison with the FACS-isolated microglia (right). The black line indicates the y = x line and the blue line is the linear fit line with slope equal to 0.71 and 0.83, respectively and CCC equal to 0.81 and 0.75, respectively
