Extended Data Fig. 8: Morphological and electrophysiological properties of control and TS t-hCO neurons.
From: Maturation and circuit integration of transplanted human cortical organoids
a–c. Morphological properties of control and TS t-hCO neurons. a–b. Examples of 3D-reconstructed t-hCO neurons derived from control Line identities of filled cells from left to right, Control: 1208-2; 2242-1; 8119-1; 2242-1; 2242-1; 1208-2; and TS: 8303-S3; 8303-S3; 7643-6; 7643-6; 9862-2; 8303-S3; 8303-S3; 8303-S3; 8303-S3; 9862-2; 8303-S3; 7643-6. c. Quantification of the soma diameter, number of primary branches, total number of dendrites and total length (control: n = 19 neurons, TS: n = 23 neurons, P = 0.592, *P = 0.025; control: n = 19 neurons, TS: n = 21 neurons P = 0.7627, * P = 0.0112). d. Comparison of membrane capacitance in hCO and t-hCO (hCO: n = 25 neurons, t-hCO: n = 18 neurons, ***P < 0.0001). e–g. Electrophysiological properties of control and TS t-hCO neurons. e. Example traces of a single AP firing in control and TS t-hCO neurons, showing differences in AP height (black arrows) and threshold (gray dashed line). f. Comparison of electrophysiological properties in control and TS t-hCO neurons; membrane capacitance (Control: n = 30 neurons, TS: n = 36 neurons, P = 0.0848), resting membrane potential (Control: n = 31 neurons, TS: n = 33 neurons, **P < 0.0044), maximal firing rate (Control: n = 29 neurons, TS: n = 36 neurons, ***P < 0.0001), spike amplitude (Control: n = 31 neurons, TS: n = 35 neurons, ***P < 0.0001), spike half-width (Control: n = 31 neurons, TS: n = 34 neurons, P = 0.0984) and spike threshold (Control: n = 31 neurons, TS: n = 34 neurons, ***P < 0.0001). g. Cumulative frequency plots and quantification of spontaneous EPSCs decay time and charge (Control: n = 32 neurons; TS: n = 26 neurons, P = 0.0744, P = 0.4812). Data are presented as mean ± SEM.
