Extended Data Fig. 6: Bone marrow chimera mice recapitulated the phenotype of Areg^ΔILC2 mice during Trichuris muris infection.

Irradiated CD45.1 WT mice received BM from Areg^fl/– or Areg^ΔILC2 donor mice. After 8 weeks, chimera mice were infected with 200 Trichuris eggs by oral gavage and analyzed 19 days post-infection. a, Experimental schematic. b-d, AREG deletion in ILC2s (b), ST2^– CD4⁺ T cells (c), and ST2⁺ CD4⁺ T cells (d) in Trichuris-infected Areg^fl/– BM (n = 5 mice) and Areg^ΔILC2 BM (n = 5 mice). e-g, Representative AB-PAS-stained images of cecal tips (bars = 100 µm) (e). Enumeration of cecal tip goblet cells (f) and crypt length (g) of naïve Areg^fl/– BM (n = 4 mice) or Areg^ΔILC2 BM (n = 3 mice) and Trichuris-infected Areg^fl/– BM (n = 5 mice) or Areg^ΔILC2 BM (n = 5 mice). h, Worm burden in Areg^fl/– BM (n = 5 mice) or Areg^ΔILC2 BM (n = 5 mice). i-l, Intestinal immune responses in cecum of Trichuris-infected Areg^fl/– BM (n = 4 mice) and Areg^ΔILC2 BM (n = 5 mice). Frequencies of ILC2s (i), ST2⁺ Th2 cells (j), and eosinophils (k), and percentages of IL-4⁺ and IL-13⁺ cecal ILC2s and CD4⁺ T cells (l). m-o, Gene expression analysis of proximal colons isolated from naïve Areg^fl/– BM (n = 4 mice) and Areg^ΔILC2 BM (n = 3 mice) and Trichuris-infected Areg^fl/– BM (n = 10 mice) and Areg^ΔILC2 BM (n = 10 mice). Normalized to Actb. Relative expression levels of pro-inflammatory genes (Il4, Il5, Il13, Ifng) and immunoregulatory responses (Il10 and Tgfb1) (m). Ratio of type 1 gene (Ifng) over type 2 gene (Il13) expression (n). Expression levels of goblet cell functional marker gene Retnlb (o). Data in b-d, f-h, i-l are representative of two independent experiments. Data in m-o are pooled from two independent experiments. Unpaired two-sided t-test (b-d, h-l). One-way ANOVA with Tukey multiple comparisons test (f, g, m-o). P values are presented where appropriate. NS, not significant. Data are represented as means ± s.e.m.

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