Extended Data Fig. 1: NMUR1-eGFP is highly expressed and enriched in ILC2s in lymphoid and non-lymphoid tissues.
From: Neuropeptide regulation of non-redundant ILC2 responses at barrier surfaces
a, Percentages of surface NMUR1+ ILC2s isolated from the indicated tissues from Nmur1+/+ WT mice (n = 5 mice). b, Schematic of targeting construct for generating Nmur1iCre-eGFP mice. c–k, Comprehensive analysis of NMUR1-eGFP expression in Nmur1iCre-eGFP mice. Representative gating strategy for live CD45+ cells (c), innate lymphoid cell (ILC) subsets (d), adaptive lymphocytes (e), and myeloid cells and granulocytes (g). Lin1: CD11b, CD11c, FcεRIα, B220. Lin2: CD3ε, CD5. Representative overlaid histograms depicting NMUR1-eGFP expression in adaptive lymphocytes (f) and myeloid cells and granulocytes (h) from Nmur1iCre-eGFP mice and Cre-negative littermate controls. Percentages of NMUR1-eGFP+ cells within the indicated immune cell subsets isolated from small intestines (i), mesenteric lymph nodes (MLN) (j), and spleens (k) of Nmur1iCre-eGFP mice (n = 3 mice). Data in a and i–k are representative of two independent experiments. Data are represented as means ± s.e.m.
