Extended Data Fig. 2: ILC2-enriched eGFP expression and iCre-mediated induction of RFP expression.
From: Neuropeptide regulation of non-redundant ILC2 responses at barrier surfaces
a, Representative gating strategy. b, Representative histograms depicting NMUR1-eGFP expression in the indicated progenitor immune cell subsets in the bone marrow. c, Percentages of NMUR1-eGFP+ cells within the indicated progenitor cells isolated from Nmur1iCre-eGFP mice (n = 4 mice). Lin1: Ly6G (1A8), CD3ε, CD5, CD19. Lin2: Ly6G (Gr1), FcεRIα. d, Unbiased analysis of NMUR1-eGFP+ cells in the bone marrow by flow cytometry, depicting a representative of two independent experiments. Lin3: CD3ε, CD5, CD19. Lin4: Ly6G (Gr1), FcεRIα. e, Percentages of RFP+ ILC2s in indicated tissues from Nmur1iCre-eGFPROSA26LSL-RFP mice (n = 4 mice). f–m, Representative histograms showing iCre (RFP) expression in the indicated immune cell subsets isolated from the small intestine (f), colon (h), MLN (j), and spleen (l) of Nmur1iCre-eGFPROSA26LSL-RFP mice (n = 4 mice). Percentage of iCre (RFP)+ cells within the indicated immune cell subsets isolated from the small intestine (g), colon (i), MLN (k), and spleen (m). Data in c,e,g,i,k,m are pooled from of 2 independent experiments. Data are represented as means ± s.e.m. MLN - mesenteric lymph node, DC – dendritic cells.
