Extended Data Fig. 1: Dimeric TMC-1 complex from C. elegans copurifies with additional proteins.
From: Structures of the TMC-1 complex illuminate mechanosensory transduction
a, Spectral confocal image of mVenus fluorescence in an adult tmc-1::mVenus worm showing mVenus fluorescence in the head neurons, cilia and body wall muscles. Shown is one representative image of five total images. b, Representative FSEC profile of the TMC-1 complex, detected via the mVenus tag. Inset shows a silver-stained, SDS-PAGE gel of the purified TMC-1 complex. Red asterisk indicates TMC-1. The experiments were repeated two times with similar results. Thyroglobulin (669 kDa) and apo ferritin (443 kDa) were used for protein molecular mass standards. c, The distribution of mVenus photobleaching steps for the TMC-1 complex is consistent with a binomial function (grey bars) an assembly with two fluorophores. A total of n = 600 spots were analysed from three photobleaching movies (200 spots per movie) at random locations in the imaging chamber. Each movie is represented by a blue dot. d, Images are shown for the SEC-purified mVenus-tagged TMC-1 complex captured with biotinylated anti-GFP nanobody. Scale bar = 5 µm. e, Representative trace showing the two-step photobleaching (red arrows) of the mVenus-tagged TMC-1 complex.
