Extended Data Fig. 5: Photodamage of EnT1.0 and EnT1.3.

To investigate the effects of photodamage, aliquots of 30 μM enzyme in PBS (pH 7.4) were pre-irradiated for 90 min (10 s on/off pulse at 365 nm) at 4 °C, before performing reactions. Control aliquots of enzyme were incubated in the dark at 4 °C. a, Bar chart showing the conversion of 1 to 1a and 1b achieved by variants DA_20_00, EnT1.0 and EnT1.3 that had been pre-irradiated with light (+) and had been incubated in the dark (−). Reaction conditions: 15 μM catalyst, 400 μM 1, 30 min irradiation (10 s on/off pulse) at 365 nm, 4 °C, PBS (pH 7.4) with 5% DMSO as a cosolvent. Error bars represent the standard deviation of measurements made in triplicate. b, SDS-PAGE analysis of protein variants (DA_20_00, EnT1.0, EnT1.3) that had been pre-irradiated with light (+) and had been incubated in the dark (−).