Extended Data Fig. 7: Total turnover numbers achieved by EnT1.3.
From: A designed photoenzyme for enantioselective [2+2] cycloadditions
Reaction conditions: 800 μM 1, 10 s on/off pulse at 365 nm, at 4 °C, in 1 ml PBS (pH 7.4) with 10% DMSO as a cosolvent, at a specified catalyst loading. A time course providing the reaction conversion of 1 to 1a and 1b at 0.2 mol% and 1.5 mol% catalyst loading (1.6 μM (red) and 12 μM (blue), respectively). A background correction has been applied to both curves, corresponding to less than 10% of turnovers. Error bars represent the standard deviation of measurements made in triplicate (error bars are not shown when they are smaller than the data-point marker).
