Extended Data Fig. 8: Evolution trajectory.
From: A designed photoenzyme for enantioselective [2+2] cycloadditions
Reaction conditions: 10 μM catalyst, 400 μM 1, 30 min irradiation (10 s on/off pulse) at 365 nm, 4 °C, PBS (pH 7.4) with 5% DMSO as a cosolvent in glass vials. a, Bar chart showing conversion of 1 to 1a and 1b and e.e. for the major product, (-)-1a, achieved by selected variants along the evolutionary trajectory. Error bars represent the standard deviation of measurements made in triplicate (error bars are not shown when they are smaller than the data-point marker). b, Table providing conversion and selectivity data for Extended Data Fig. 8a.
