Extended Data Fig. 4: Immune cell profiling of lungs and peripheral blood of APOE knock-in mice with COVID-19.
From: Common human genetic variants of APOE impact murine COVID-19 mortality
a–b, Gating strategy to delineate leukocyte subsets (a) and assessment of the proportion of leukocyte subsets (b) in dissociated lungs of APOE knock-in mice on day 4 post infection with SARS-CoV-2 MA10 (n = 21, 15, 20 for APOE2, APOE3, and APOE4, respectively; data pooled from two independent experiments; P values according to one-tailed t-tests). c, Gating strategy to delineate leukocyte subsets in peripheral blood of APOE knock-in mice with COVID-19. d–g, Concentration of CD45+ leukocytes (d) and proportion of myeloid (e) and lymphoid (f) subsets in the peripheral blood of APOE knock-in mice on day 4 post infection with SARS-CoV-2 MA10 as assessed by flow cytometry (n = 10, 9, 7 for APOE2, APOE3, and APOE4, respectively; P values according to two-sided t tests). g, Representative flow cytometry plots for (e-f). Boxplot whiskers in b and d-f extend to the smallest and largest value within 1.5 × interquartile ranges of the hinges, and box centre and hinges indicate median and first and third quartiles, respectively.
