Extended Data Fig. 6: TANGO2 transfers haem in vitro.
From: HRG-9 homologues regulate haem trafficking from haem-enriched compartments
a, Quantification of ZnMP fluorescence in wild type, hrg-9 and hrg-10 double knockout worms, and the double knockout worms expressing TANGO2 genes from human (hTANGO2), yeast (ytango2), or zebrafish (ztango2). n = 30 worms examined over 3 independent experiments. Data are presented as mean ± s.e.m. P values were determined by one-way ANOVA followed by Tukey’s test. b,c, Haem transfer from mitochondria isolated from mouse liver to apo-myoglobin in the absence or presence of yeast Tango2p (b) and zebrafish Tango2 (c). d,e, Haem transfer assays for human TANGO2, BSA (d), and GFP (e). Mitochondria isolated from mouse liver were used as the haem donor and apo-myoglobin was used as the haem acceptor. f,g, Incorporation of 10 μM (f) and 1 μM (g) haem into apo-myoglobin in the absence or presence of TANGO2. h, Haem transfer from the mitochondria isolated from TANGO2-knockout HEK293 cells to apo-myoglobin in the absence or presence of TANGO2. i, Coomassie blue staining validating the protein digestion by trypsin in mitochondrial membranes isolated from mouse liver. j, Haem transfer from protein-free mitochondrial membranes to apo-myoglobin in the absence or presence of TANGO2. Mito, mitochondria.
