Extended Data Fig. 7: STING–AP-1 interaction depends on TBK1-mediated phosphorylation.
From: Clathrin-associated AP-1 controls termination of STING signalling
a, HeLa STING KO cells transfected with FLAG-tagged STINGWT or STINGLR(L374A/I375A) were treated with 2.5 µM diABZI for 0, 1 or 2 h, immunoprecipitated with anti-FLAG antibody, and analysed by western blot. b, HeLa TBK1 KO cells reconstituted with an empty plasmid or with plasmids expressing TBK1WT or enzyme-dead TBK1S172A were treated with 2.5 µM diABZI for 2 h and analysed by western blot. GAPDH was used as a processing control. c, HeLa cells pretreated with DMSO or 2 µM BX795 for 24 h were stimulated with 2.5 µM diABZI or not (2 h) and analysed by western blot. Vinculin was used as a loading control. One representative of at least two (a–c) independent experiments is shown. Ratios of target proteins versus loading control normalized to the untreated sample of each condition (b,c). d, Mass spectrometry detected molecular weight of SUMO LBD-STING and TBK1-phosphorylated LBD-STING (pSTING). e, Bio-layer interferometry binding studies of LBD-STINGELI(E360A/L364A/I365A) with AP-1 ΔμCTD. f, Bio-layer interferometry binding studies of LBD-STING3S(S355D/S358D/S366D) with AP-1 ΔμCTD. One representative of at least two (e, f) independent experiments is shown.
