Extended Data Fig. 8: Cryo-EM analysis of pSTING in complex with AP-1.
From: Clathrin-associated AP-1 controls termination of STING signalling
a, Purification and SDS–PAGE analysis of AP-1 core in complex with pSTING and ARF1. b, Purification and SDS–PAGE analysis of AP-1 core in complex with STING3S (S355D/S358D/S366D). c, Representative micrograph of AP-1 pSTING complex in vitrified ice from 30,004 raw images. Scale bar, 30 nm. d, 2D class averages of AP-1 pSTING complex particles. Box size, 27 nm. e, Flow chart of data processing; see Methods for details. f, Final 3D reconstruction of the AP-1 pSTING complex, coloured according to the local resolution. g, 3D Fourier shell correlation of final 3D reconstruction of the AP-1 pSTING complex. h, Corrected Gold-standard Fourier shell correlation curves of the AP-1 pSTING complex for the 3D electron microscopy reconstruction. i, Angular distribution of the AP-1 pSTING particles included in the final reconstruction. One representative of at least two (a,b) independent experiments is shown.
