Extended Data Fig. 5: AP-1 depletion boosts STING-dependent immune activation in different cell types.
From: Clathrin-associated AP-1 controls termination of STING signalling
a,b, HaCaT cells (a) or THP-1 cells (b) transfected with NC siRNA or AP-1 siRNA for 3 days were treated with 2.5 µM diABZI for indicated time and analysed by western blot. GAPDH was used as a loading control. c,d, Human primary epithelial cells incubated with NC siRNA or AP-1 siRNA for 3 days were treated with 2.5 µM diABZI and were analysed by RT–qPCR (c) and western blot (d). Vinculin was used as a loading control. e–j, Fibroblast cells derived from patients with SAVI characterized by STING1 N154S mutation (e,f), H72N (g,h) or V147M (i,j) were incubated with NC siRNA or AP-1 siRNA for three days and then analysed by western blot. Vinculin was used as a loading control in f,h,j. e,g,i, Induction of IFNB1, IFIT1, IFIT2, IFIT3 expression was assessed by RT–qPCR in HeLa cells transfected with NC siRNA or AP-1 siRNA for 3 days and then stimulated with 1 µM diABZI. Mean ± s.d. of three (c,e,g,i) technical replicates. P values based on two-tailed Student’s t-tests (c,e,g,i).
