Fig. 4: H2B.8 is localized in AT-rich TEs and transcriptionally inactive intergenic regions.
From: Histone H2B.8 compacts flowering plant sperm through chromatin phase separation
a, Genome snapshots of H2B.8 abundance in sperm (log2(IP/input)), sperm cell transcription (log2(RPKM)) and gene and TE annotations (orange, euchromatic TE; magenta, heterochromatic TE) at representative regions. RPKM, reads per kilobase of transcript per million mapped reads. b, Profiles of sperm H2B.8 enrichment over genes (grouped by sperm cell expression), TEs (grouped by chromatin state) and intergenic regions. c,f, Scatterplots showing anticorrelation of sperm H2B.8 enrichment with sperm transcription (c) or GC content (f) among indicated genomic features. ρ, Spearman’s rank. Colours are as d. d, Coverage of genes, euchromatic TEs and heterochromatic TEs (left y axis, 500-kb windows) and H2B.8 enrichment in sperm (right y axis; 1-kb windows) along chromosome 1. Chromosomes 2–5 are shown in Extended Data Fig. 4a. e, PCA of H2B.8 abundance in p35S::H2B.8–eGFP seedlings with other chromatin marks. The green and pink shaded areas represent euchromatic and heterochromatic marks, respectively.
