Extended Data Fig. 8: Unsupervised clustering of the infected hepatocytes based on Plasmodium transcriptome.

a, UMAP colored by hours post-infection (hpi) and inoculation method. b, UMAP colored by Abortive/Productive tag assigned to cells based on clustering analysis in Fig. 3a. c, UMAP colored by Louvain cluster (Methods). d, Heatmap for marker genes per cluster. purple – low expression; yellow – high expression. e, The contribution of different sampled time points to the composition of individual clusters and the complete infected hepatocytes atlas. g, Total Plasmodium (PBA) reads increase with time in productive clusters. Y axis - log10 of the sum of PBA reads. Pairwise significance between clusters was determined by two-sided Wilcoxon rank-sum test (ns:p > 0.05; ****:p < = 0.0001; ELS-MELS:p < 2.22e-16, MELS-MLLS:p < 2.22e-16, MLLS-LLS:p = 0.15, LLS-Abortive:p = 3.9e-7; n cells per cluster from left-to-right: 1,657/1,434/507/319/198). bounds of boxes span IQR, horizontal black lines denote the median, whiskers span 1.5*IQR (Interquartile range), black-bold circles indicate datapoints outside of said range.