Extended Data Fig. 1: TNKS2 SAM-PARP polymerisation promotes auto-PARylation.
From: Structural basis of tankyrase activation by polymerization
Recombinant TNKS2 PARP and SAM-PARP were analysed for auto-PARylation by Western blotting, either before or after in vitro PARylation by incubation with NAD+. Y920ASAM, polymerisation-deficient mutant variant; G1032WPARP, catalytically inactive mutant variant. Top, anti-pan-ADP-ribose Western blot; bottom, Revert-stained membrane for protein load. One representative out of three independent experiments with similar results is shown.
