Extended Data Fig. 5: Sleep/wakefulness of Lkb1 and Sik3 gene-modified mice.
From: Kinase signalling in excitatory neurons regulates sleep quantity and depth
a. In vitro kinase assay for SIK3, SIK3 (T221A) and SIK3 (T221E). b–d. Total wake time (b), NREMS episode duration (c), total REMS time (d) of Syn1creERT; Lkb1flox mice (+/+, n = 9; flox/flox, n = 14). e. NREMS delta power for 2 h after 6-hour sleep deprivation of Syn1creERT; Lkb1flox mice (+/+, n = 8; flox/flox, n = 10). f–i. Total wake time (f), NREMS episode duration (g), total REMS time (h), and NREMS delta power under the basal condition and after 6-hour sleep deprivation (i) of Sik3 mice (+/+, n = 11; T221A/+, n = 12). j–l. Total wake time (j), NREMS episode duration (k), and total REMS time (l) of Syn1creERT; Lkb1flox; Sik3 mice (+/+, n = 14; T221E/T221E, n = 15). m–o. Total wake time (m), NREMS episode duration (n), and total REMS time (o) of Sik3 mice (Slp/+, n = 7; Slp-T221A/+, n = 10). p. NREMS delta power under the basal condition and after 6-hour sleep deprivation of Sik3 mice (Slp/+, n = 6; Slp-T221A/+, n = 10). Data shown as mean ± s.e.m.; two-sided P values. Two-way ANOVA (c, f, g, h, k, o) with Tukey’s test (b, d, j, l–n); two-way repeated measures with Sidak’s test (e, i, p).
