Extended Data Fig. 9: Sik3Slp in excitatory neurons increased NREMS.
From: Kinase signalling in excitatory neurons regulates sleep quantity and depth
a–f. NREMS episode duration of Vglut2cre; Sik3Slp-flox mice (a)(+/+, n = 19; Slp-flox/+, n = 17; Slp-flox/Slp-flox, n = 20), Vglut1cre; Sik3Slp-flox mice (b)(+/+, n = 11; Slp-flox/+ n = 11; Slp-flox/Slp-flox, n = 10), Camk2acre; Sik3Slp-flox mice (c)(+/+, n = 11; Slp-flox/+, n = 13; Slp-flox/Slp-flox, n = 10), Vgatcre; Sik3Slp-flox mice (d)(+/+, n = 10; Slp-flox/+, n = 13; Slp-flox/Slp-flox, n = 12), Foxg1cre; Sik3Slp-flox mice (e)(+/+, n = 17; Slp-flox/+, n = 12; Slp-flox/Slp-flox, n = 14), and Foxd1cre; Sik3Slp-flox mice (f)(+/+, n = 27; Slp-flox/+, n = 16; Slp-flox/Slp-flox, n = 21). g–l. Total wake time of Vglut2cre; Sik3Slp-flox mice (g), Vglut1cre; Sik3Slp-flox mice (h), Camk2acre; Sik3Slp-flox mice (i), Vgatcre; Sik3Slp-flox mice (j), Foxg1cre; Sik3Slp-flox mice (k), and Foxd1cre; Sik3Slp-flox mice (l). Colours are as for a–f, respectively. m-r. Total REMS time of Vglut2cre; Sik3Slp-flox mice (m), Vglut1cre; Sik3Slp-flox mice (n), Camk2acre; Sik3Slp-flox mice (o), Vgatcre; Sik3Slp-flox mice (p), Foxg1cre; Sik3Slp-flox mice (q), and Foxd1cre; Sik3Slp-flox mice (r). Colours are as for a–f, respectively. s, t. Relative NREMS delta power changes after sleep deprivation to each ZT under the basal condition of Vglu2cre; Sik3Slp-flox mice (s)(+/+, n = 13; Slp-flox/Slp-flox, n = 16) and Foxd1cre; Sik3Slp-flox mice (t)(+/+, n = 10; Slp-flox/Slp-flox, n = 11). u–z. Process S simulation for sleep need dynamics. Td/Ti ratio of Hdac4SA mice (u)(+/+, n = 10; SA/+, n = 13), Hdac5SA mice (v)(+/+, n = 9; SA/+, n = 13; SA/SA, n = 7), Vglut2cre; Sik3flox mice (w)(+/+, n = 10; flox/flox, n = 17), Vglut1cre; Sik3flox mice (x)(+/+, n = 10; flox/flox, n = 12), Vglut2cre; Sik3Slp-flox mice (y)(+/+, n = 16; Slp-flox/Slp-flox, n = 13), and Foxd1cre; Sik3Slp-flox mice (z)(+/+, n = 11; Slp-flox/Slp-flox, n = 10). Data shown as mean ± s.e.m.; two-sided P values. Two-way ANOVA with Tukey’s test when applicable (a–r); mixed-effects model (s, t); unpaired t-test (u, w–z); one-way ANOVA with Tukey’s test (v).
