Extended Data Fig. 10: Single-nucleus RNA-seq analysis of the cerebral cortex transduced with AAV-Sik3^Slp and of sleep-deprived mice.

a. The number of detected reads in nuclei of WPRE-positive cells of each cluster from the cerebral cortex transduced with AAV-Sik3^Slp or AAV-Sik3. b. The number of detected reads in nuclei of each cluster from the cerebral cortex of sleep-deprived or ad libitum sleep mice. c, d. The number of genes detected in nuclei of each cluster from the cerebral cortex transduced with AAV-Sik3^Slp or AAV-Sik3 (c), and sleep-deprived or ad libitum sleep mice (d). e. WPRE-positive nuclei were displayed on UMAP of nuclei from the cerebral cortex transduced with AAV-Sik3^Slp or AAV-Sik3 (6 mice per group). f. Volcano plot of genes in the GABAergic cluster of nuclei from the cerebral cortex transduced with AAV-Sik3^Slp or AAV-Sik3. The vertical axis indicates -log₁₀(adjusted P value) of Wilcoxon rank-sum test with Bonferroni correction. The horizontal axis indicates log₂(fold change) calculated from AAV-Sik3^Slp compared with AAV-Sik3. Each point represents a gene detected in at least 10% of nuclei in any conditions. Black circles, P ≥ 0.05 or |log₂(fold change)|≤0.14. Red circles, P < 0.05 and log₂(fold change) > 0.14. Blue circles, P < 0.05 and log₂(fold change) < −0.14. g–j. Volcano plots of genes in the GABAergic cluster (g), astrocyte cluster (h), oligodendrocyte cluster (i), and microglia cluster (j) of nuclei from the cerebral cortex of sleep-deprived (n = 4) or ad libitum sleep mice (n = 6). k, l. Expression level of Bdnf in nuclei from the cerebral cortex transduced with AAV-Sik3^Slp or AAV-Sik3 (k) and the cerebral cortex of sleep-deprived or ad libitum sleep mice (l).