Extended Data Fig. 3: Increased angiogenesis during progression from papilloma to SCC.
From: Ras drives malignancy through stem cell crosstalk with the microenvironment
a, UMAP of the examples of angiogenesis-associated mRNAs from scRNA analyses of SCCs that show enrichment in the C2 signature (see Fig. 1c for annotation of clusters). b, Collapsed z-stack rendering of clearing and whole-mount immunofluorescence of tissue sections (n > 8 tumours per stage). Keratin 14 labels the tumour epithelium; CD31 labels the vasculature. Quantifications are at right. Note that the blood vessel proximity is closer in invasive SCC than papilloma. Scale bars, 40 µm. (n = 8 tumours per condition per stage, p(0–25) = 0.0034, p(25–50) = 0.0801, p(50–75) = 0.7548, p(75–100) = 0.4734). All statistics were using unpaired two-tailed Student’s t test: ns, p ≥ 0.05); *, p ≤ 0.05); **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. Data are presented as mean ± s.e.m.
