Extended Data Fig. 2: Filopodia in L2/3 pyramidal neurons exhibit AMPAR immune-negative and NMDAR immune-positive synapses.
From: Filopodia are a structural substrate for silent synapses in adult neocortex
a, Example confocal image of a V1 L2/3 neuron (green arrowhead) expressing GFP after viral transfection in V1 in an originally 45 μm thick slice. Scale bar: 100 μm expanded/ 59 μm original. Image was taken after reshrinking the tissue from 4x expansion to 1.7x expansion. b, Box plot (left) and kernel density estimate (right) of signal intensity in Bassoon (blue), NMDAR (yellow), and AMPAR (red) channels for L2/3 pyramidal neuron spines (n = 275). Box plot represents median and IQR with whiskers extending to the most extreme points not considered outliers. Signal in each channel is shown for all dendritic protrusions, each represented by one dot. c, As in b, but for filopodia (n = 134).
