Extended Data Fig. 4: Representatitive examples of eMAP at dendritic protrusions.
From: Filopodia are a structural substrate for silent synapses in adult neocortex
Example four channel images of dendritic protrusions with different dhead/dneck values (increasing from left to right). From top to bottom: cell-filling GFP stained with Alexa Fluor 488 (green) at lower magnification to show full protrusion shape, presynaptic protein Bassoon stained Alexa Fluor 405 (blue), NMDAR subunit NR1(GluN1) stained with Alexa Fluor 555 (yellow), and AMPAR subunit GluR1(GluA1) stained with Alexa Fluor 647 (red), all at higher magnifiaction to show synaptic localization. Scale bar: 2 μm expanded/0.5 μm original.
