Extended Data Fig. 8: Analyses of ELPVs, spheroids and amyloid plaques in 5-month-old 5xFAD mice with PLD3 overexpression.
From: PLD3 affects axonal spheroids and network defects in Alzheimer’s disease
a, Confocal images of spheroids with GFP or PLD3 overexpression. Right panels show zoomed images from dashed boxes. b, Spheroid sizes in 5-month-old 5xFAD mice with PLD3 or GFP overexpression, presented by individual mice (b) or PAAS (b’). N = 6 and 5 mice for PLD3 and GFP groups, respectively; each dot represents average measurements from 350~600 PAAS. Violin plots show distributions of 1400~1600 individual PAAS from each group. c, Confocal images of adjacent PAAS with (blue dashed line) and without (yellow dashed line) PLD3 overexpression. Arrows indicated enlarged ELPVs. d, ELPV occurrence in PAAS in 5-month-old 5xFAD mice with PLD3 or GFP overexpression. N = 4 mice for each group. Each dot represents average measurements from 150~250 PAAS. e and f, Plaque number (e) and size (f) in mice with GFP or PLD3 overexpression. N = 5 and 6 for GFP and PLD3 groups, respectively. For (f), each dot represents average measurements from 100~250 plaques. g, Confocal images of LAMP1-positive vesicular structures in PAAS and cell bodies in 10-month-old mice with PLD3 overexpression. h, ELPV sizes in groups described in g. N = 4 mice for each group. Each dot represents average measurements from 500~1000 ELPVs from PAAS or 100–200 LAMP1-positive vesicles from cell bodies. i, Confocal images of spheroids and LAMP1-positive vesicles in wildtype mice overexpressing PLD3. Two-tailed Mann-Whitney tests were performed in b, b’ and d. Two-tailed unpaired t-tests were performed in e and f. Two-tailed Welch’s t-test was used in h. Data are represented as mean ± S.E.M.
