Extended Data Fig. 6: AS TCR tetramer staining on SCT transfectants.
From: Autoimmunity-associated T cell receptors recognize HLA-B*27-bound peptides
a. Representative flow cytometry showing AS8.4 cells alone, AS8.4 T cells + K562 (HLA-B*27:05+), AS8.4 T cells + K562 (HLA-B*27:05+ GPER1+), AS8.4 T cells + K562 (HLA-B*27:05+ YEIH+) and AS8.4 T cells + K562 (HLA-B*27:05+ 1uM YEIH peptide). b. Plots of percentage of CD69% positive AS8.4 T cells alone, AS8.4 T cells + K562 (HLA-B*27:05+), AS8.4 T cells + K562 (HLA-B*27:05+ GPER1+), AS8.4 T cells + K562 (HLA-B*27:05+ YEIH+) and AS8.4 T cells + K562 (HLA-B*27:05+ 1uM YEIH peptide). Data are shown as mean±s.d., n = 3 biological replicates. NS, not significant, P = 0.5453; ****P<0.0001 (one-way ANOVA). c. Representative flow cytometry histograms showing AS TCR tetramer staining for SCT transfected b2m KO 293T cells. Red histograms: negative controls (irrelevant p-HLA-B*27:05 construct); Blue histograms: potential arthritogenic peptide identified in this study.
