Extended Data Fig. 4: Selection of AS TCRs on HLA-B*27:05 libraries.
From: Autoimmunity-associated T cell receptors recognize HLA-B*27-bound peptides
a. HLA-B*27:053mut 10mer library enrichment per round of selection by the five AS TCRs as measured by flow cytometry. b. AS4.1, AS4.2, AS4.3 and AS4.4 TCR tetramer staining on the 4th round HLA-B*27:05 9-AA libraries. HLA-B*27:053mut expression was monitored by anti-C-Myc-488. Streptavidin-647 (SA-647) was used to tetramerize and fluorescently label the AS TCRs. c. AS3.1, AS4.1, AS4.2, AS4.3 and AS4.4 TCR tetramer staining on the 4th round HLA-B*27:053mut 10-AA libraries. HLA-B*27:05 expression was monitored by anti-HA-488. Streptavidin-647 (SA-647) was used to tetramerize and fluorescently label the AS TCRs. d. WebLogos representing the unique 4th round selected peptides for each AS TCR based on deep sequencing reads. The size of each amino acid letter represents its abundance at the given position among the unique peptides. e. Heatmap plots showing the amino acid composition per position of the peptides enriched after the 4th round of selection. Darker color represents greater abundance of a given amino acid at specific position.
