Extended Data Fig. 5: Postmitotic and interphase assembly are spatially distinguished for the first hour after mitotic exit and thus their kinetics can be decomposed.
From: A quantitative map of nuclear pore assembly reveals two distinct mechanisms
a, Time-lapse 3D imaging of Nup93-mEGFP genome-edited cell. Single confocal sections of SiR-DNA and GFP channels are shown. Images were filtered with a median filter (kernel size: 0.25 × 0.25 μm). Scale bar, 10 μm. Time after AO is indicated. b, The fluorescence intensities at non-core (brown) and inner-core (yellow) regions were quantified. Dots represent the average and s.d. of measurements from 14 cells. The intensities were fitted with a sequential model of NPC assembly (bold lines) that allows for different population and rate constants for postmitotic and interphase assembly (described in detail in Methods and Supplementary Table 3). c,d, Decomposed kinetics of postmitotic (c) and interphase (d) assembly from (b).
