Extended Data Fig. 2: Immunoblot analysis of mEGFP-Nup205 (a,b) and mEGFP-Nup358 (c,d) cell lysates.
From: A quantitative map of nuclear pore assembly reveals two distinct mechanisms
The membranes were cut at the position of around 200 kDa. The membranes above 200 kDa were blotted with antibodies against Nup205 (a) and Nup358 (c), and the membranes below 200 kDa were blotted with an antibody against Vinculin for loading control. The clone 081 (a) and 097 (c) are the cell lines that are used in this study. (b,d) The relative intensity of the bands for GFP-tagged Nups over endogenous Nups. The plots for Nup205 and Nup358 are from 9 and 8 pairs of bands (GFP-tagged vs endogenous) from 4 and 3 independent experiments, respectively. The mean is depicted as a horizontal line and the whiskers show the standard deviation.
