Extended Data Fig. 4: GFP-tagged Nups are recruited to the NPCs rather than nonspecifically accumulated on the NE.
From: A quantitative map of nuclear pore assembly reveals two distinct mechanisms
a, Three-dimensional stimulated emission depletion (3D-STED) imaging of a Nup62-mEGFP genome-edited cell at early telophase. The region of the nuclear envelope indicated by a white line in the top image is flattened and shown in the bottom. Scale bars, 1 μm. The density of the Nup62 spots was 14.6 ± 2.6 NPCs/μm2 (from 6 cells), which is consistent with our previous EM observation of the NPC density of 12–16 NPCs/μm2 in early telophase cells11. b, 2D-STED imaging of a mEGFP-Nup107 genome-edited cell at early telophase that were stained with anti-GFP and anti-Elys antibodies. Enlarged images indicated by white boxes in the top images are shown in the bottom panel. Scale bars, 1 μm.
