Fig. 3: Paternally inherited DNA damage is mainly repaired by TMEJ.

a, The distribution of translocation footprints in fog-2 F₁ adults with paternal exposure to 90 Gy ionizing radiation. Templated inserts are insertions of ≥3 bp in between the fusion sites that have a matching sequence within ±25 bp around one of the 2 break points. Miscellaneous insertions are insertions <3 bp or insertions with no matching sequence within ±25 bp around the breakpoints. n, number of translocations. b, Left, schematic illustration of the type 1 translocations. The sense strand is in blue, and the antisense strand is in red. Numbers indicate the distance of the nucleotide to the breakpoint; yellow boxes indicate positions that show microhomology. Right, heat map representing the sum of all type 1 translocation maps derived from ∆mf and ∆mm (n = 23 translocations), excluding translocations with a templated insertion. Darker shades indicate higher sequence similarity between the corresponding bases. Numbers along the x- and y-axes correspond to those on the left. P values are provided in Supplementary Table 1. c, Progeny lethality of indicated strains. n represents the number of biological replicates. Data are median ± 95% confidence interval. P values are shown. d, Progeny lethality of different parental combinations for polq-1 worms with and without exposure to 90 Gy ionizing radiation. n = 3 biological replicates. Data are median ± 95% confidence interval. P values are shown. GLM with logit link function and Tukey multiple comparisons were used for proportional data, and one-way (c) or two-way (d) ANOVA with arcsine transformation was used to confirm the statistical results. Full statistical analyses are provided in Supplementary Table 1.