Fig. 5: Depletion of histone H1 or HPL-1 triggers HRR to alleviate transgenerational lethality.
From: Inheritance of paternal DNA damage by histone-mediated repair restriction
a, RAD-51 immunofluorescence (red) and DAPI staining (blue) in the germlines of EV (RNAi); fog-2, his-24 (RNAi); fog-2 and hpl-1 (RNAi); fog-2 worms with or without paternal exposure to 90 Gy ionizing radiation. Top, pachytene zone of the female germlines. Bottom, transition zone of male germlines. Scale bar, 10 μm. Experiment in a was repeated three times with similar observations. b, Quantification of RAD-51 foci per nucleus in the germline of females and males, with or without paternal exposure to 90 Gy ionizing radiation. More than 15 nuclei were counted for each germline; n represents the number of germlines. Each dot shows the mean of the number of RAD-51 foci per nucleus in one germline. Bars show median ± 95% confidence interval. P values are shown; nested one-way ANOVA with Bonferroni’s multiple comparisons test, reflecting the variation between different germlines. c, Progeny lethality characterization of empty vector, his-24 and hpl-1 RNAi on fog-2 and brc-1; fog-2 mutants with or without paternal ionizing radiation irradiation (90 Gy). Control, ∆m: n = 3; ∆mf, ∆mm: n = 5 biological replicates. Data are median ± 95%confidence interval. P values are shown; GLM with logit link function and Tukey multiple comparisons; one-way ANOVA with arcsine transformation was used for the proportional data to confirm the statistical results and are included in the full statistical results (Supplementary Table 1).
