Extended Data Fig. 7: H2AZ phenocopies BRD8 in maintaining GBM proliferation.

a, Western blot showing depletion efficiency of H2AZ using two independent sgRNAs (sgH2AZ-1 and sgH2AZ-2) compared to a negative control (sgNeg). HSC70 serves as loading control. b, GFP dropout assays of sgNeg, sgCDK1 (a positive control), and sgH2AZ-1 and sgH2AZ-2 in A382^WT cells. Plotted is the mean ± s.d. (n = 3 biologically independent samples). c, RT-qPCR assays of the indicated cell cycle and senescence-related genes after transduction of sgNeg, sgBRD8-1 and sgBRD8-2, or sgH2AZ-1 and sgH2AZ-2 in A382^WT cells. Plotted is the mean ± s.d. (n = 3 biologically independent samples). P values were calculated using two-tailed unpaired Student’s t-tests. d, SA-β-gal assays of A382^WT cells transduced with sgNeg, sgBRD8-2, or sgH2AZ-2. Scale bar, 50 µm. Data shown represents three independent results. e, GSEA plots after H2AZ depletion in A382^WT cells using the upregulated (left) and the downregulated (right) gene signatures generated from RNA-seq profiling after BRD8 loss. f, GFP dropout assays of sgNeg, sgCDK1, sgRNAs targeting EP400 and H2AZ in the indicated cell lines. Plotted is the mean ± s.d. (n = 3 biologically independent samples). g, SA-β-gal assays in Ctrl or p53-deficient A382^WT cells transduced with sgNeg, sgBRD8-2, or sgH2AZ-2. Data shown represents three independent results. Scale bar, 50 µm. h, RT-qPCR assays of indicated cell cycle and senescence-related genes after transduction of sgNeg, sgBRD8-2, or sgH2AZ-2 in Ctrl and p53-deficient A382^WT cells. Plotted is the mean ± s.d. (n = 3 biologically independent samples). P values were calculated using two-tailed unpaired Student’s t-tests. i, GFP dropout assays of sgNeg, sgCDK1, and sgH2AZ-1 in Ctrl or two p21-deficient cells. Plotted is the mean ± s.d. (n = 3 biologically independent samples). P values were calculated using two-tailed unpaired Student’s t-tests.