Extended Data Fig. 5: BRD8 is a subunit of the EP400 complex in GBM.
From: BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network
a, Western blot and silver staining of proteins immunoprecipitated by anti-Flag antibody in Flag-tagged BRD8-overexpressing or empty vector control (Ctrl) A382WT cells. Data shown represents three independent results. b, Summary of peptides detected by IP-MS in Flag-tagged BRD8 or Ctrl immunoprecipitates for 17 subunits of the EP400 complex. c, IP and western blots showing validation of core subunits of the EP400 complex in BRD8 immunoprecipitates. Data shown represents two independent results. HSC70 serves as loading control. d, Summary of specific and shared components of the EP400 and SRCAP complexes. Proteins pulled down by BRD8 are shown in dark blue text. e, RT-qPCR showing expression levels of EP400 and SRCAP in A382WT GBM cells. Plotted represents three biological replicates. f, Summary of GSEA analysis using P53OE up signature (genes significantly upregulated after p53 induction by doxycycline) after individual depletion of multiple components of the EP400 complex. Two independent sgRNAs were used for targeting each individual subunit, and sgNeg was used as control. g, Summary of GSEA analysis upon depletion of indicated components of the EP400 complex using the sgBRD8 up signature (genes significantly upregulated after BRD8 depletion by two independent sgRNAs and each sgRNA has two biological replicates. h, Schematic of the EP400 complex that occupies p53 target loci in GBM, with BRD8 being the most specific regulator.
