Extended Data Fig. 9: Confocal laser scanning micrographs of E. coli BL21(AI) transformed with SuCas12a2–crRNA and LbCas12-crRNA expression plasmids under target and non-target conditions in the absence of antibiotic selection. | Nature

Extended Data Fig. 9: Confocal laser scanning micrographs of E. coli BL21(AI) transformed with SuCas12a2–crRNA and LbCas12-crRNA expression plasmids under target and non-target conditions in the absence of antibiotic selection.

From: Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA

Extended Data Fig. 9: Confocal laser scanning micrographs of E. coli BL21(AI) transformed with SuCas12a2–crRNA and LbCas12-crRNA expression plasmids under target and non-target conditions in the absence of antibiotic selection.

Extensive filamentation can be observed with the bacteria containing the target-expression plasmid and expressing SuCas12a2 and target crRNA 2 h and 4 h after induction. Within each overlaid image, fluorescent DNA (DAPI) staining is shown in blue and membrane (FM4–64) staining is shown in red. Paired images are representative of 2 independent experiments.

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