Extended Data Fig. 5: Exposure to PA14 does not induce AWA expression of several previously identified receptors for pheromone sensing.
From: Pathogenic bacteria modulate pheromone response to promote mating
a–g, Sample images (a–f) and quantification (g) for srg-36p::gfp, daf-37p::gfp, srd-1p::gfp, srx-44p::gfp, srbc-64p::gfp, and srbc-66p::gfp expression in AWA neurons in adult hermaphrodites exposed to OP50 or PA14 for 4–6 h (reporters are indicated by the name of the promoters in g). Arrows point to cell bodies of several sensory neurons. Lines outline worm bodies. Scale bar (applicable to images in the same row), 20 μm. A, anterior. D, dorsal (arrows point out of the page for OP50 condition in c and for PA14 condition in d). Intensity of fluorescence signals is normalized using average intensity of AWA expression of str-44p::gfp in OP50-exposed worms measured in parallel. In box plots, the centre line shows the median, box edges delineate 1st and 3rd quartiles and whiskers extend to minimum and maximum values. Numbers in parentheses, number of different neurons (g). Dots, signals of individual neurons (g). P values are derived from Two-way ANOVA with Bonferroni’s multiple comparisons test, asterisks indicate significant difference, **** P < 0.0001; ns, not significant. P values are shown in Source data.
