Extended Data Fig. 1: Time of day of engraftment dictates tumour size.
From: Dendritic cells direct circadian anti-tumour immune responses
(a) Tumour volume of each mouse after engraftment of B16-F10-OVA cells at 6 different times of the day (Zeitgeber time (ZT)); n = 10 mice per timepoint from 2 independent experiments. (b) Tumour volume after engraftment of E0771 cells at 2 different times of the day, plotted per group (left) and mouse (right); n = 5 mice from 2 independent experiments, two-way ANOVA. (c-d) Normalized tumour volume, plotted per group (left) and mouse (right) (c) and tumour volume on day 20 (d), after engraftment of 4T1 cells at 2 different times of the day; n = 12 (ZT9), n = 10 (ZT21) mice from 2 independent experiments, two-way ANOVA (c) and unpaired Student’s t-test (d). (e) Normalized tumour volume after engraftment of MC-38 cells at 2 different times of the day, plotted per group (left) and mouse (right); n = 8 (ZT9), n = 6 (ZT21) mice from 2 independent experiments, two-way ANOVA. (f) Fluorescence flux in photons/seconds of B16-F10-OVA-Luc (5 × 105 cells) tumours on day 5 after engraftment; n = 8 (ZT9), n = 7 (ZT21) mice from 2 independent experiments, unpaired Student’s t-test. (g) Tumour volume in phase-shifted mice injected using the same batch of B16-F10 cells (without OVA expression); n = 9 (ZT9), n = 6 (ZT21) mice from 2 independent experiments, two-way ANOVA. (h) Scheme of the normal (light:dark), inverted lighting (dark:light) and jet lag protocols. For the jet lag, every three days mice were placed into a 6 h- or 12 h-phase delayed environment. The red dots represent the time when tumours were engrafted (9 h after the start of the cycle). All data are represented as mean ± SEM, all t-tests are two-tailed.
