Extended Data Fig. 8: A-Eos co-localize with CD4+ T cells in human IBD.
From: Active eosinophils regulate host defence and immune responses in colitis
a, Regulon activity in A-Eos across conditions (n = 4, Il5-tg). b, Multidimensional scaling (MDS) plot of bulk RNA-seq samples shown in Fig.4a. c, Heat map of signature gene expression across conditions of samples shown in Fig. 4a. d, A-Eos (PD-L1+CD80+) frequencies upon treatment of BM-Eos with IL-33 and/or IFNγ. (n = 4, B6J). Data represents mean ± SEM. One-way ANOVA. e, EPX immunofluorescence of A-Eos upon exposure to IFNγ for 90 min. Splenic eosinophils were magnetically enriched (n = 2, Il5-tg), treated overnight with colon CM and A-Eos sorted by flow cytometry. Scale bar, 10 µm. f, Frequencies of PD-L1+ and CD80+ in colonic eosinophils of WT (n = 6, B6J) and Eo-Cre;Ifngrfl/fl mice (n = 4) upon C. rodentium infection, relative to uninfected controls (n = 2, B6J). Medians are shown. Two-tailed unpaired Student’s t-test. g, Left: UMAP of single-cell eosinophil transcriptomes isolated from the colon of anti-IFNγR-treated, C. rodentium-infected or control Il5-tg mice (n = 3). Middle: expression of IFNγ target genes. Right: Expression of granule and antimicrobial signatures. Data represents mean ± SD. Two-sided Wilcoxon test. h, Observed vs. expected number of contacts between clusters of SIGLEC8 and CD4 molecules shown per slide. P Values are computed based on a two-sided permutation test (see Methods). i, Proportions of segmented cells expressing SIGLEC8 only (blue) or co-expressing both SIGLEC8 and CD4 (red) across slides. Dotted horizontal line shows mean. j, Mean count per slide of molecules of a given transcript in the proximity (<10 µm) of SIGLEC8 RNA molecules spatially associated with CD4 molecules vs SIGLEC8 molecules not associated with CD4 molecules. The central line in the box plot represents the median count per slide, the lower and upper hinge corresponds to the first quartiles and the whisker extends from the hinge to the smallest or largest value no further than 1.5 x IQR from the hinge. Two-sided paired Wilcoxon test (17 ROIs, n = 4 patients).
