Extended Data Fig. 7: Chromatin dynamics during annelid development.

a, Genomic feature annotation of the consensus ATAC-seq peaks. b, Stacked bar plots showing the proportion of called peaks per developmental stage classified by genomic feature. c, Heatmap of normalised peak accessibility of the soft clustered consensus ATAC-seq peak sets. d, Self-correlation matrices of normalised motif accessibility and transcription factor binding score, revealing distinct chromatin regulatory dynamics throughout development. e, Correlation matrices of normalised motif accessibility to transcription factor binding score during annelid development. f, Correlation matrix of normalised motif accessibility between both species. d–f further validate the non-triviality of the results obtained in Fig. 3c. Pearson correlation coefficients in d–f were derived from two-tailed tests. g, Heatmap of normalised motif accessibility and transcription factor binding dynamics for each of the common annotated annelid motif archetypes during O. fusiformis and C. teleta development. Colour scale denotes transcription factor binding score dynamics, bubble size represents motif accessibility dynamics, both in a z-score scale. Motif archetypes highlighted in red are representative examples of the heterochronic shifts shown in bulk in Fig. 3c. h, Enrichment analysis of the number of occurrences of the common annotated annelid motif archetypes in the peak clusters inferred through soft k-means clustering and shown in c, for O. fusiformis (top) and C. teleta (bottom). For each cluster and motif combination, the Bonferroni-adjusted p–value of the two-tailed Fisher’s exact test is shown. Red cells represent significantly overrepresented lineages (odds ratio, OR > 1, adjusted p–value < 0.05). Blue cells denote significantly underrepresented lineages (OR < 1, adjusted p–value < 0.05). i, Most abundant bound motifs in peaks of the Hox clusters. j, Downstream regulated genes by transcription factors bound to the HOX/CDX/EVX motif archetype.