Fig. 1: Reconstitution and structure of the Raptor–TFEB–Rag–Ragulator complex.
From: Structure of the lysosomal mTORC1–TFEB–Rag–Ragulator megacomplex
a, Size-exclusion chromatography and SDS–PAGE of assembled Raptor–TFEB–Rag–Ragulator complex. Peak-1 corresponds to the fully assembled complex, and peak-2 represents Ragulator alone. All the corresponding bands are labelled, the asterisk indicates HSP70 contamination. MW, molecular weight; A280, absorbance at 280 nm. b, Domain arrangement of all the subunits in the complex. Unresolved domains are indicated by dashed lines. c, A composite cryo-EM density map of the complex, assembled from three focused-refinement maps (Raptor, c-RagAGTP/RagCGDP–Ragulator and TFEB-nc–RagAGTP/RagCGDP–Ragulator). Different contour levels were used for optimal visualization using UCSF ChimeraX46. c, canonical; nc, non-canonical.
