Extended Data Fig. 5: Assembly of Raptor-TFEB1–109-Rag-Ragulator complex.
From: Structure of the lysosomal mTORC1–TFEB–Rag–Ragulator megacomplex
a, SDS-PAGE analysis of the purified TFEB1–109-Rag complex before TEV cleavage and gel filtration. b, Gel filtration chromatography of the Raptor-Rag-Ragulator and Raptor-TFEB1–109-Rag-Ragulator complexes, using a Superose 6 10/300 GL (GE Healthcare) column. The peaks corresponding to the largest complex are analyzed by SDS-PAGE and stained by Coomassie blue.
