Extended Data Fig. 8: Cryo-EM workflow of the mTORC1-TFEB-Rag-Ragulator megacomplex.
From: Structure of the lysosomal mTORC1–TFEB–Rag–Ragulator megacomplex
a, Cryo-EM data processing diagram of the mTORC1-TFEB-Rag-Ragulator megacomplex. b, A representative micrograph of the dataset after motion correction. c, SDS-PAGE of the reconstituted megacomplex stained by Coomassie blue. Asterisk indicates HSP70 contamination. d, Selected 2D class average images showing different orientations of the complex. e, Resolution plots of the asymmetric unit of mTORC1-TFEB-Rag-Ragulator megacomplex after symmetry expansion. f, Orientation distribution of local refinement after symmetry expansion of the mTORC1-TFEB-Rag-Ragulator complex. Scale bars in b and d represent 20 nm.
