Fig. 1: ALOX15 is required for AM maintenance.
From: Neonatal imprinting of alveolar macrophages via neutrophil-derived 12-HETE
a, Representative FACS plots (left) and quantification (right) of AM numbers (gated on single live cells, CD45.2+CD11c+Siglec-F+) in the lungs of adult WT (n = 7) and Alox15−/− (n = 6) mice. b, AM numbers in BAL (n = 9 mice per group). c, Quantification of the AM population in the lungs of BM chimeras 8 weeks after reconstitution (left to right, n = 5, 5, 8, 11, 11, 9 and 5 mice per group, respectively). d, Model of AM development. e–g, Numbers of fetal monocytes (e), pre-AMs (f) and AMs (g) in WT and Alox15−/− lungs at various ages (n = 6 (PND0) or 8 (PND1 and PND3) per group). h, Top upregulated and downregulated pathways from KEGG Pathway enrichment analysis. i, Violin plot (left) and heatmap (right) of mRNA transcripts significantly upregulated and downregulated in the cell cycle pathway (n = 3 (WT) or 4 (Alox15−/−)). Data are presented as the mean ± s.e.m. and are from one experiment (h,i) or pooled from two (a,c,e–g) or three (b) independent experiments. Data were analysed using unpaired two-tailed t-test (a,b), Mann–Whitney two-tailed test (i) or one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test (c). The model in d was created using BioRender (https://biorender.com).
