Extended Data Fig. 5: Cryo-EM processing pipeline and analysis for S5_CTP dataset. | Nature

Extended Data Fig. 5: Cryo-EM processing pipeline and analysis for S5_CTP dataset.

From: Structural basis for substrate selection by the SARS-CoV-2 replicase

Extended Data Fig. 5: Cryo-EM processing pipeline and analysis for S5_CTP dataset.The alternative text for this image may have been generated using AI.

(a) Cryo-EM processing pipeline for the S5_CTP dataset. (b) Angular distribution plot for the S5_CTP dataset, calculated in cryoSPARC. Scale depicts number of particles assigned to a specific angular bin. (c) Nominal 2.67 Å resolution cryo-EM reconstruction filtered by local resolution and colored according to fitted model chain. Right panel is clipped to reveal RTC active site. (d) Directional 3D FSC for S5_CTP, determined with 3DFSC. (e) Gold-standard FSC plot for the S5_CTP dataset, calculated by comparing two half maps from cryoSPARC. The black dotted line represents the 0.143 FSC cutoff.

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