Extended Data Fig. 10: Prolonged LPS stimulation increases mitochondrial membrane potential and dsRNA.
From: Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
a, MFI of TMRM staining in BMDMs (n = 3). Immunofluorescence (b) and quantification (c) of dsRNA in BMDMs (n = 8 (0/48 h); n = 9 (24 h)). n = technical replicates from representative experiment. Data are mean ± s.d. Scale bar = 20 μm. d, Ddx58 and Ifih1 expression in BMDMs (n = 4; LPS 4 h; Ddx58 (P = 0.0000000010), Ifih1 (P=0.00000012)). e, Fh1 expression in IFN-β-stimulated BMDMs (n = 3). a,d,e, Data are mean ± s.e.m. b, 1 representative image of 3 experiments shown. n = biological replicates unless stated otherwise. P values calculated using two-tailed Student’s t-test for paired comparisons, one-way ANOVA for multiple comparisons.
